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Anti -GAD65 Rabbit pAb

價(jià)格：在線咨詢

貨號(hào)：

KT011492

產(chǎn)品規(guī)格：

100 μL

Applications：

Species：

H,M,R

品牌：

萬(wàn)物

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Product Information

Description	GAD65 rabbit polyclonal antibody
Protein full name	Glutamate decarboxylase 2
Synonyms	GAD2, GAD65, Glutamate decarboxylase 2, 65 kDa glutamic acid decarboxylase, DCE 2, Glutamic Acid Decarboxylase 2, Glutamic Acid Decarboxylase 65
Immunogen	Recombinant protein corresponding to Mouse GAD65
Isotype	IgG
Purity	Affinity purification
Predicted MW.	65 kDa
Observed MW.	65 kDa
Uniprot ID	Q05329, Q05683, P48320

Applications

Applications	Species	Dilution	Positive Sample
WB	Human, Mouse, Rat	1: 500-1: 1000	brain, cerebellum, hippocampus

Background

Glutamate decarboxylase or glutamic acid decarboxylase (GAD) is an enzyme that catalyzes the decarboxylation of glutamate to GABA and CO2. GAD uses PLP as a cofactor. In mammals, GAD exists in two isoforms with molecular weights of 67 and 65 kDa (GAD67 and GAD65). GAD67 and GAD65 are expressed in the brain where GABA is used as a neurotransmitter, and they are also expressed in the insulin-producing β-cells of the pancreas. GAD65, synthesizes GABA for neurotransmission, and therefore is only necessary at nerve terminals and synapses. In order to aid in neurotransmission, GAD65 forms a complex with Heat Shock Cognate 70 (HSC70), cysteine string protein (CSP) and Vesicular GABA transporter VGAT, which, as a complex, helps package GABA into vesicles for release during neurotransmission.

Images

Western blot analysis of GAD65 (GB11562) at dilution of 1: 500

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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